Brand new SH QTL into chromosome step three was also consistent with the QTLs getting En ce qui concerne (More document step 1: Dining table S2)

A separate venue on the chromosome seven for SH is actually imagined by the examination the people on screenhouse

To your people Kalarata/NSIC Rc238, a maximum of four significant QTLs towards chromosomes 3, 5, six, eight, and you can 8 have been seen to possess Sur significantly less than one another examination criteria (Table 2). Around three high QTLs was basically recognized having SH on chromosomes step 1, step 3, and 7, where QTLs towards chromosomes 1 and you will step three had been understood from inside the one another screening criteria.

The brand new Sur QTLs qSUR5–1 (named as qSUR5–step 1

The tolerant parent Kalarata contributed the SUR QTLs qSUR3–1_{Rc238-SCR-fourteen}, qSUR6–1_{Rc238-SCR-fourteen}, and qSUR7–1_Rc238-SCR-14 while the QTLs qSUR5–1_{Rc238-SCR-fourteen} and qSUR8–1 _{Rc238-SCR-fourteen} came from susceptible parent NSIC Rc238 under screen house condition at 14 DAS (Table 2). Same five QTLs showed effect on survival at 14 DAS under tray-on-table condition and at 21 DAS in both screening conditions. The largest effect QTL qSUR7–1 (named https://hookupranking.com/ as qSUR7–1_{Rc238-SCR-fourteen}, qSUR7–1_Rc238-TAB-14, qSUR7–1_Rc238-SCR-21 and qSUR7–1_Rc238-TAB-21) showed a LOD score of to and explained % to % of the phenotypic variation, with an additive effect of % to %, followed by qSUR6–1 (named as qSUR6–1_Rc238-SCR-14, qSUR6–1_Rc238-TAB-14, qSUR6–1_Rc238-SCR-21 and qSUR6–1_Rc238-SCR-21), which explained % to % of the phenotypic variation, detected by a LOD score of 5.97 to 6.96, and had an additive effect of 9.79% to%. qSUR3–1 (named as qSUR3–1_Rc238-SCR-14, qSUR3–1_{Rc238-TAB-fourteen}, qSUR3–1_Rc238-SCR-21 and qSUR3–1_Rc238-TAB-21) contributed % to % of the phenotypic variation, with an additive effect of % to %. _Rc238-SCR-14, qSUR5–1_{Rc238-TAB-fourteen}, qSUR5–1_Rc238-SCR-21 and qSUR5–1_Rc238-TAB-21) and qSUR8–1 (named as qSUR8–1_Rc238-SCR-14, qSUR8–1_Rc238-TAB-14, qSUR8–1_Rc238-SCR-21 and qSUR8–1_Rc238-TAB-21) from high-yielding susceptible parent NSIC Rc238 contributed 9.76% to % and % to % of the phenotypic variation, with LOD scores of 4.10 to 4.57 and 4.78 to 5.21, respectively (Table 2, Additional file 1: Figure S2).

All SH QTLs were contributed by Kalarata, the parent with anaerobic germination potential. The largest effect QTL identified for SH is qSH1–1 (named as qSH1–1_Rc238-SCR-21 and qSH1–1_Rc238-TAB-21), which is consistent over both screening conditions. It explained a phenotypic variation of 12.5% to 22.1%, with a LOD score of 5.4 to 9.4. The second largest effect QTL detected for SH was consistent with SUR QTL qSH3–1 (named as qSH3–1_{Rc238-SCR-fourteen}, qSH3–1_Rc238-SCR-21 and qSH3–1_Rc238-TAB-21,), which was also consistent in both screening conditions. This QTL explained phenotypic variation of 10.1% to14.4%, with a LOD score of 3.7 to 5.8 and an additive effect of 0.84% to 5.17%. QTL qSH7–1_Rc238-SCR-21 was identified with a LOD score of 4.6 and explained 10.7% of the phenotypic variation and had an additive effect of 4.57%. It was identified only under screenhouse conditions and was located in a different position from the identified QTL (qSUR7–1) for SUR (Additional file 1: Table S2, Additional file 1: Figure S3A). Under controlled conditions, no QTL was detected for germination, whereas one QTL on chromosome 1 (qSH1-2_Rc238-CON-21) was detected for SH with an LOD score of 6.1 that could explain 14.1% of the phenotypic variation (Additional file 1: Table S2). Altogether, the identified QTLs for SUR explained phenotypic variation in the range of % to % while that for the trait SH ranged from 10.1% to 36.5% for the Kalarata/NSIC Rc238 population.

Toward people Kalarata/NSIC Rc222, all in all, about three extreme QTLs for the chromosomes 3, six, and you may 7 have been seen to own Sur around both testing standards, which have been along with similar to the first inhabitants (Desk step three). Several extreme QTLs were recognized to possess SH on chromosomes step 1 and step three, where the QTL for the chromosome step 1, perceived not as much as both screening criteria, was also common with the initial people for the very same feature. Throughout instances, parent Kalarata which have anaerobic germination potential provided new knowledgeable alleles for AG (Even more file step 1: Dining table S3).